The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis

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dc.creator Hernández-Pando, Rogelio
dc.creator Barrios-Payán, Jorge
dc.creator Mata-Espinosa, Dulce
dc.creator Marquina-Castillo, Brenda
dc.creator Hernández-Ramírez, Diego
dc.creator Bottasso, Oscar
dc.creator Bini, Estela Isabel
dc.date.accessioned 2015-08-03T13:35:24Z
dc.date.available 2015-08-03T13:35:24Z
dc.date.issued 2015-07-22
dc.identifier.issn 1932-6203 es
dc.identifier.uri http://hdl.handle.net/2133/4868
dc.description Background The high mobility group box 1 (HMGB1) is the prototype of alarmin protein released by stressed or dying cells. The redox state of this protein confers different functions in the regulation of inflammation and immune response. Aim Determine the kinetics, cellular sources and function of HMGB1 in experimental tuberculosis. Methods BALB/c mice were infected with Mycobacterium tuberculosis strain H37Rv. At different time points, HMGB1 was quantified in bronchial lavage fluid (BALF) and in lungs was determined its cellular sources by immunohistochemistry. HMGB1 was blocked with specific antibodies or recombinant HMGB1 was administered during early or late infection. Bacilli burdens, inflammation and cytokines expression were determined. Results The maximal concentration of HMGB1 in BALF was at day one of infection. Bronchial epithelium and macrophages were the most important sources. At day 7 to 21 the oxidized HMGB1 was predominant, while during late infection only the reduced form was seen. Blocking HMGB1 during early infection produced significant decrease of bacilli burdens and high production of pro-inflammatory cytokines, while the opposite was seen when HMGB1 was administered. Blocking HMGB1 activity or administrated it in high amounts during late infection worsening the disease. Conclusions HMGB1 is liberated during experimental tuberculosis and promotes or suppress the immune response and inflammation depending on the redox state. es
dc.description.sponsorship Estela Isabel Bini was awarded with a travelling fellowship from DMM Company of Biologists, England. This work was supported by the Bilateral International Project Argentina/México, CONICET/CONACYT, number 190527. es
dc.format application/pdf
dc.format.extent 1-14 es
dc.language.iso eng es
dc.publisher PLOS (Public Library of Science) es
dc.rights openAccess es
dc.rights http://creativecommons.org/licenses/by/4.0/
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.subject Antibodies es
dc.subject Inflammation es
dc.subject Cytokines es
dc.subject Macrophages es
dc.subject Mycobacterium tuberculosis es
dc.subject Immunostaining es
dc.subject Enzyme-linked immunoassays es
dc.title The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder © 2015 Hernández-Pando et al. es
dc.relation.publisherversion http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133200 es
dc.rights.text This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. es
dc.citation.title PLOS ONE es
dc.description.fil Fil: Bottasso, Oscar. Institute of Experimental and Clinic Immunology, Rosario, IDICER, CONICET, School of Medical Sciences. UNR. Rosario; Argentina es
dc.description.fil Fil: Bini, Estela Isabel. Institute of Experimental and Clinic Immunology, Rosario, IDICER, CONICET, School of Medical Sciences. UNR. Rosario; Argentina es
dc.type.collection articulo
dc.type.version publishedVersion es


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